Once a Chromosomal Position for a Putative Gene Associated with Parkinson

Once a chromosomal position for a putative gene associated with Parkinson disease is verified, which of the following is a next step?

A) ascertaining all living and at- risk individuals who are family members of the affected test subjects
B) fine mapping of the linkage region to identify particular variants for influencing risk
C) developing a FISH screening test to identify pre- Parkinson patients
D) determining whether this locus is mutated in all Parkinson patients
E) using a genome- wide linkage SNP panel to identify more minor genes

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