The Rate of an Enzyme-Catalyzed Reaction Initially Increases with an Increase

The rate of an enzyme-catalyzed reaction initially increases with an increase in the substrate concentration,but eventually reaches a maximum value,even though the concentration of substrate continues to increase.Which of the following best explains why?

A) As substrate concentration increases,the substrates preferentially bind with each other instead of the active site of the enzyme,and no additional catalysis occurs.
B) As substrate concentration increases,the active sites of all the enzyme molecules become occupied with substrate molecules,and no additional binding can occur.
C) An increase in substrate concentration eventually increases the temperature of the reaction mixture,and the enzyme becomes denatured.
D) An increase in substrate concentration allows for greater competition between substrate and inhibitor binding,and enzyme activity ceases.
E) When substrate concentrations become too high,local variations of pH occur that cause the enzyme to become denatured.

Correct Answer:

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