What Do You Predict Would Happen If You Created a TRNA

What do you predict would happen if you created a tRNA with an anticodon of 5?-CAA-3? that is charged with methionine, and added this modified tRNA to a cell-free translation system that has all the normal components required for translating RNAs? $\text { The Genetic Code }$
$\begin{array}{|l|l|l|l|l|}\hline & \text { U } & \text { C } & \text { A } & \text { G } \\\hline &\text { UUU Phe (F) } & \text { UCU Ser (S) } & \text { UAU Tyr (Y) } & \text { UGU Cys (C) } \\&\text { UUC - } & \text { UCC - } & \text { UAC - } & \text { UGC - } \\ \text { U } &\text { UUA Leu (L) } & \text { UCA -- } & \text { UAA Stop } & \text { UGA Stop } \\&\text { UUG - } & \text { UCG - } & \text { UAG Stop } & \text { UGG Trp (W) } \\\hline &\text { CUU Leu (L) } & \text { CCU Pro (P) } & \text { CAU His (H) } & \text { CGU Arg (R) } \\&\text { CUC - } & \text { CCC - } & \text { CAC - } & \text { CGC - } \\ \text { C}&\text { CUA - } & \text { CCA - } & \text { CAA Gin (Q) } & \text { CGA - } \\&\text { CUG }- & \text { CCG }- & \text { CAG }- & \text { CGG }- \\\hline &\text { AUU Ile (I) } & \text { ACU Thr (T) } & \text { AAU Asn (N) } & \text { AGU Ser (S) } \\&\text { AUC - } & \text { ACC - } & \text { AAC - } & \text { AGC - } \\ \text { A}&\text { AUA - } & \text { ACA - } & \text { AAA Lys (K) } & \text { AGA Arg (R) } \\&\text { AUG Met (M) } & \text { ACG - } & \text { AAG - } & \text { AGG - } \\\hline &\text { GUU Val (V) } & \text { GCU Ala (A) } & \text { GAU Asp (D) } & \text { GGU Gly (G) } \\&\text { GUC - } & \text { GCC - } & \text { GAC - } & \text { GGC - } \\ \text { G}&\text { GUA - } & \text { GCA - } & \text { GAA Glu (E) } & \text { GGA - } \\&\text { GUG - } & \text { GCG - } & \text { GAG - } & \text { GGG - } \\\hline\end{array}$ Table 7-29

A) Methionine would be incorporated into proteins at some positions where glutamine should be.
B) Methionine would be incorporated into proteins at some positions where leucine should be.
C) Methionine would be incorporated into proteins at some positions where valine should be.
D) Translation would no longer be able to initiate.

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