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You Have Produced a Monoclonal Antibody That Binds to the Protein

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You have produced a monoclonal antibody that binds to the protein actin.To be sure that the antibody does not cross-react with other proteins, you test your antibody in a western blot assay on whole-cell lysates that have been subjected to electrophoresis under nondenaturing conditions (shown in Figure 4-69A) and denaturing conditions (shown in Figure 4-69B).Does the antibody cross-react with other proteins? If so, does this explain the results in the two western blots? If not, how do you explain the difference observed? You have produced a monoclonal antibody that binds to the protein actin.To be sure that the antibody does not cross-react with other proteins, you test your antibody in a western blot assay on whole-cell lysates that have been subjected to electrophoresis under nondenaturing conditions (shown in Figure 4-69A) and denaturing conditions (shown in Figure 4-69B).Does the antibody cross-react with other proteins? If so, does this explain the results in the two western blots? If not, how do you explain the difference observed? Figure 4-69
Figure 4-69

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No, the antibody does not seem to cross-...

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