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Nested PCR Is Very Sensitive and Specific Because

Question 41

Multiple Choice

Nested PCR is very sensitive and specific because


A) it uses more than one DNA polymerase to make three to four times the amount of PCR products than standard PCR.
B) the assay itself basically serves as a form of internal control and ensures specificity.
C) the assay will act as a form of external control, ensuring specificity and sensitivity.
D) the fluorochrome that is attached to the primers ensures specificity of the nucleotides that are attached to the template.

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