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The Double-Reciprocal Transformation of the Michaelis-Menten Equation,also Called the Lineweaver-Burk

Question 27

Multiple Choice

The double-reciprocal transformation of the Michaelis-Menten equation,also called the Lineweaver-Burk plot,is given by
1/V0 = Km /(Vmax[S]) + 1/Vmax
To determine Km from a double-reciprocal plot,you would:


A) multiply the reciprocal of the x-axis intercept by -1.
B) multiply the reciprocal of the y-axis intercept by -1.
C) take the reciprocal of the x-axis intercept.
D) take the reciprocal of the y-axis intercept.
E) take the x-axis intercept,where V0 = 1/2 Vmax.

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