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Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Edition 6ISBN: 978-1305577206Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Edition 6ISBN: 978-1305577206 Exercise 21
The enzyme alcohol dehydrogenase catalyzes the oxidation of ethyl alcohol by NAD + to give acetaldehyde plus NADH and a proton:
CH3 CH2 OH + NAD + ? ? CH3 CHO + NADH + H +
The rate of this reaction can be measured by following the change in pH. The reaction is run in 1 ml 10 mM TRIS buffer at pH 8.6. If the pH of the reaction solution falls to 8.4 after ten minutes, what is the rate of alcohol oxidation, expressed as nanomoles of ethanol oxidized per sec per ml of reaction mixture?
CH3 CH2 OH + NAD + ? ? CH3 CHO + NADH + H +
The rate of this reaction can be measured by following the change in pH. The reaction is run in 1 ml 10 mM TRIS buffer at pH 8.6. If the pH of the reaction solution falls to 8.4 after ten minutes, what is the rate of alcohol oxidation, expressed as nanomoles of ethanol oxidized per sec per ml of reaction mixture?
Explanation
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A mole of H + is produced for every mole...
Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
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