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Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Edition 6ISBN: 978-1305577206Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Edition 6ISBN: 978-1305577206 Exercise 17
Plot the titration curve for Bicine and calculate how to prepare a pH 7.5 Bicine buffer solution Bicine (N, N-bis (2-hydroxyethyl) glycine) is another commonly used buffer in biochemistry labs. The structure of bicine in its fully protonated form is shown below:
a. Draw the titration curve for Bicine, assuming the pK a for its free COOH group is 2.3 and the pK a for its tertiary amino group is 8.3.
b. Draw the structure of the fully deprotonated form (completely dissociated form) of bicine.
c. You have available a 0.1 M solution of Bicine at its isoelectric point (pHI), 0.1 M solutions of HCl and NaOH, and ample distilled H2O. Describe the preparation of 1 L of 0.04 M Bicine buffer, pH 7.5.
d. What is the concentration of fully protonated form of Bicine in your final buffer solution?
a. Draw the titration curve for Bicine, assuming the pK a for its free COOH group is 2.3 and the pK a for its tertiary amino group is 8.3.
b. Draw the structure of the fully deprotonated form (completely dissociated form) of bicine.
c. You have available a 0.1 M solution of Bicine at its isoelectric point (pHI), 0.1 M solutions of HCl and NaOH, and ample distilled H2O. Describe the preparation of 1 L of 0.04 M Bicine buffer, pH 7.5.
d. What is the concentration of fully protonated form of Bicine in your final buffer solution?
Explanation
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a) The titration curve for Bicine will h...
Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
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