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Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Edition 6ISBN: 978-1305577206Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Edition 6ISBN: 978-1305577206 Exercise 16
How to prepare a buffer solution: a Tris buffer solution
Tris -hydroxymethyl aminomethane (TRIS) is widely used for the preparation of buffers in biochemical
research. Shown here is the structure TRIS in its protonated form:
Its acid dissociation constant, K a , is 8.32 x 10 -9. You have available at your lab bench a 0.1 M solution of TRIS in its protonated form, 0.1 M solutions of HCl and NaOH, and ample distilled water. Describe the preparation of a 1 L solution of 0.02 M TRIS buffer, pH 7.8.
Tris -hydroxymethyl aminomethane (TRIS) is widely used for the preparation of buffers in biochemical
research. Shown here is the structure TRIS in its protonated form:
Its acid dissociation constant, K a , is 8.32 x 10 -9. You have available at your lab bench a 0.1 M solution of TRIS in its protonated form, 0.1 M solutions of HCl and NaOH, and ample distilled water. Describe the preparation of a 1 L solution of 0.02 M TRIS buffer, pH 7.8.
Explanation
Verified
Verified
The protonated form of TRIS will only ex...
Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
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